Nucleic acid quantitation from tissue slides
US7968327B2 · kind B2 · utility
15Cited by
11References
20Claims
0Family size
Inventors
Key dates
| Filing date | Aug 14, 2007 |
| Grant date | Jun 28, 2011 |
| Priority date | — |
| Expiry date | Aug 14, 2027 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/143333
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
This invention provides methods of quantitating nucleic acids from problematic samples, such as aged samples, formalin fixed samples, paraffin embedded samples, samples with aneuploid cells, and cells with fragmented nucleic acids. Methods include techniques to efficiently solublize the nucleic acids under non-denaturing conditions from preserved clinical samples without resort to organic extractions, to normalize cell counts regardless of aneuploidy, to access the fragmentation state of the nucleic acids, and to provide standard curves for degraded nucleic acid samples.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.