Method for stabilizing assay reagents, reagent container with stabilized assay reagents and use thereof
US7972838B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jun 3, 2005 |
| Grant date | Jul 5, 2011 |
| Priority date | — |
| Expiry date | Jun 15, 2028 |
Classification
- Technology area (CPC B)Performing Operations; Transporting
- CPC primaryB01L2300/16
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A reagent container having an inner surface upon which at least two reagents are dried, with the first reagent dried on a first area separate from a second area where the second reagent is dried. The first and second reagents are a nucleic acid polymerase and its substrate. A method is disclosed which includes dispensing at least the first and second reagents onto separate areas of the inner surface of the reagent container, and removing excess water from the reagents. The reagent container can be used in a polymerase chain reaction (PCR) assay, an assay that utilizes a reverse transcriptase, a reverse transcriptase polymerase chain reaction, an immuno-PCR assay, a nucleic acid sequence based assay, a proximity ligation assay, a ligase chain reaction assay, a rolling circle amplification assay, and a strand displacement amplification assay.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.