Methods for detecting dihydroxyvitamin D metabolites by mass spectrometry

US7972868B2 · kind B2 · utility

Assignee

• Quest Diagnostics Investments LLC · US

Inventors

• Brett Holmquist · Thousand Oaks, US
• Nigel J. Clarke · Vista, US
• Anne Caston-Balderrama · Rancho Palos Verdes, US
• Richard E. Reitz · San Clemente, US

Key dates

• Filing date: Nov 28, 2007
• Grant date: Jul 5, 2011
• Expiry date: Oct 31, 2029

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10T436/24
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Provided are methods of detecting the presence or amount of a dihydroxyvitamin in D metabolite in a sample using mass spectrometry. The methods generally comprise ionizing a dihydroxyvitamin D metabolite in a sample and detecting the amount of the ion to determine the presence or amount of the vitamin D metabolite in the sample. In certain preferred embodiments the methods include immunopurifying the dihydroxyvitamin D metabolites prior to mass spectrometry. Also provided are methods to detect the presence or amount of two or more dihydroxyvitamin D metabolites in a single assay.