Methods for synthesis of encoded libraries

US7972992B2 · kind B2 · utility

Assignee

• Praecis Pharmaceuticals, Inc. · US

Inventors

• Barry Morgan · Franklin, US
• Stephen Hale · Belmont, US
• Christopher C. Arico-Muendel · Watertown, US
• Matthew Clark · Waltham, US
• Richard W. Wagner · Waterbury, US
• David I. Israel · Concord, US
• Malcolm L. Gefter · Lincoln, US
• Dennis Benjamin · Redmond, US
• Nils Jakob Vest Hansen · Copenhagen, DK
• Malcolm J. Kavarana · Fairfax, US
• Steffen Phillip Creaser · Cambridge, US
• George J. Franklin · Auburn, US
• Paolo A. Centrella · Acton, US
• Raksha Acharya · Bedford, US

Key dates

• Filing date: Dec 17, 2004
• Grant date: Jul 5, 2011
• Expiry date: Jul 6, 2026

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C40B50/10
• WIPO field: Organic fine chemistry
• WIPO sector: Chemistry

Abstract

The present invention provides methods of synthesizing a molecule comprising a functional moiety which is operatively linked to an encoding oligonucleotide. The methods include providing an initiator compound comprising an initial functional moiety comprising n building blocks, wherein the initial functional moiety comprises at least one reactive group, and is operatively linked to an initial oligonucleotide; reacting the initiator compound with a building block comprising at least one complementary reactive group, under conditions suitable for reaction of the complementary reactive group to form a covalent bond; and reacting the initial oligonucleotide with an incoming oligonucleotide corresponding to the building block in the presence of an enzyme which catalyzes ligation of the initial oligonucleotide and the incoming oligonucleotide, under conditions suitable for ligation of the incoming oligonucleotide and the initial oligonucleotide to form an encoding oligonucleotide.