Circular probe amplification (CPA) using energy-transfer primers
US7977053B2 · kind B2 · utility
1Cited by
31References
41Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | Dec 12, 2006 |
| Grant date | Jul 12, 2011 |
| Priority date | — |
| Expiry date | Mar 22, 2027 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6844
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides methods and kits for the rapid exponential amplification of nucleic acid molecules using a padlock probe. The present invention improves upon the existing methods for amplifying padlock probes by eliminating or delaying the appearance of artifact products that cause false positive results, and also increase the sensitivity and speed of the assay. Further provided are nucleic acid amplification primers containing non-informative base analogs.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.