Transcription template for cell-free protein synthesis and method using the same
US7981617B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Aug 28, 2001 |
| Grant date | Jul 19, 2011 |
| Priority date | — |
| Expiry date | Jan 27, 2026 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/686
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods to construct a transcription template for cell-free protein synthesis that has a high translation template activity, using a 3′-side primer and a 5′-side primer for PCR are provided. The 5′-side primer for PCR has a sequence complementary to a base sequence containing at least a part of a promoter functional site from the 5′-end of a promoter and has a base sequence that does not contain a base sequence complementary to at least a part of a RNA polymerase-recognizing site of the 3′-end of the promoter. The other primer has a base sequence complementary to at least a part of the RNA polymerase-recognizing site of the 3′-end of the promoter and has a sequence that does not contain a base sequence complementary to at least a part of a promoter functional site from the 5′-end of the promoter.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.