Polymeric carriers for immunohistochemistry and in situ hybridization
US7985557B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | May 22, 2008 |
| Grant date | Jul 26, 2011 |
| Priority date | — |
| Expiry date | Feb 6, 2029 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/588
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Certain disclosed embodiments of the present invention concern the synthesis, derivatization, conjugation to immunoglobulins and signal amplification based on discrete, relatively short polymers having plural reactive functional groups that react with plural molecules of interest. Reactive functional groups, such as hydrazides, may be derivatized with a variety of detectable labels, particularly haptens. The remaining reactive functional groups may be conjugated directly to a specific binding molecule, such as to the oxidized carbohydrate of the Fc region of the antibody. Disclosed conjugates display large signal amplification as compared to those based on molecules derivatized with single haptens, and are useful for assay methods, particularly multiplexed assays.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.