Alkaline proteases and detergents and cleaners comprising these alkaline proteases

US7985570B2 · kind B2 · utility

Assignee

• B.R.A.I.N. Biotechnology Research and Information Network AG · DE

Inventors

• Susanne Wieland · Nievenheim, DE
• Karl-Heinz Maurer · Erkrath, DE
• Beatrix Kottwitz · Erkrath, DE
• Frank Niehaus · Heppenheim, DE
• Patrick Lorenz · Lorsch, DE

Key dates

• Filing date: Mar 5, 2010
• Grant date: Jul 26, 2011
• Expiry date: Mar 5, 2030

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C11D3/38618
• WIPO field: Basic materials chemistry
• WIPO sector: Chemistry

Abstract

The present application relates to two novel proteases (SEQ ID NO: 4 and 7) which are similar to one another. The DNA of the two proteases, was obtained from soil samples, and C-terminally deleted, producing proteolytically active fragments thereof (SEQ ID NO: 5 and 8). The invention also provides all alkaline proteases similar at least to 90% to SEQ ID NO: 4 or to 87.5% to SEQ ID NO: 7, and those which can be summarized under a consensus sequence (SEQ ID NO: 9) derived from SEQ ID NO: 4 and 7. Furthermore, it relates to all nucleic acids which have a homology of at least 85% identity to the associated nucleic acids (SEQ ID NO: 3 and 6) or the fragments concerned. Furthermore, it defines technical possibilities of use for these proteases and especially describes their use in detergents and cleaners.