Patent · US Active

Modified nitrilases and their use in methods for the production of carboxylic acids

US7985572B2 · kind B2 · utility

0Cited by

3References

19Claims

0Family size

Assignee

BASF SE

Inventors

Thomas Zelinski · Menasha, US
Maria Keβeler · Mannheim, DE
Bernhard Hauer · Neheim, DE
Thomas Friedrich · Darmstadt, DE

Key dates

Filing date	Feb 24, 2004
Grant date	Jul 26, 2011
Priority date	—
Expiry date	Jan 12, 2027

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12P7/42
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

The present Invention relates to new nitrilases with modified Substrate acceptance, the method to obtain them and the use of the aforesaid nitrilases. Said nitrilases are encoded by a polypeptide sequence, which at a Position corresponding to Position 296 in wild-type Alcaligenes faecalis comprises an amino acid which is not tyrosine. The Invention further relates to nucleic acid sequences and amino acid sequences encoding Said nitrilases, expression constructs comprising Said nucleic acid sequences, vectors comprising the nucleic acid sequences or the exPression constructs, to organisms, preferably microorganisms, comprising the nucleic acid sequences, expression constructs or vectors. The Invention additionally relates to a method for preparing carboxylic acids, preferably substituted chiral carboxylic acids from racemic nitriles.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.