Biomolecule immobilization on biosensors

US7989396B2 · kind B2 · utility

Assignee

• The Board of Trustees of the Leland Stanford Junior University · US

Inventors

• Heng Yu · Taichung, TW
• Nader Pourmand · San Mateo, US
• Shan X. Wang · Portola Valley, US

Key dates

• Filing date: Dec 3, 2007
• Grant date: Aug 2, 2011
• Expiry date: Sep 1, 2029

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C40B40/10
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

A highly specific and versatile surface chemistry for immobilization of amine-terminated probes is disclosed. A bi-layered polymer thin film serves as the platform for coupling the probes, which are preferably oligonucleotides. The process involves sequentially coating a substrate with polyamine and polyacid anhydride. Hydrolyzed polyacid anhydride groups may be converted to non-hydrolyzed groups at about 100° C. prior to probe attachment. The process of coating the substrate requires no harsh chemical pretreatment of substrates such as RCA or Piranha cleaning. In addition, simple thermal activation of the anhydride groups has a low requirement for storage, leading to a long shelf life of modified surfaces. The disclosed surface chemistry is especially compatible with microfabrication processes, and its effective application to magnetic biosensors is demonstrated.