PCR process and arrangement for DNA amplification using dry reagents

US7993828B2 · kind B2 · utility

Assignee

• Siemens Aktiengesellschaft · DE

Inventors

• Walter Gumbrecht · Herzogenaurach, DE
• Peter Paulicka · Erlangen, DE
• Manfred Stanzel · Erlangen, DE

Key dates

• Filing date: Apr 26, 2005
• Grant date: Aug 9, 2011
• Expiry date: May 26, 2026

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/686
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A PCR process for DNA amplification with thermocyclisation of the corresponding reagents is disclosed, in which total integration of all substances and process steps is achieved in a closed, single-use unit (so-called cartridge) in which the reagents are stored in a storage-stable form at room temperature. According to the process, the water-soluble reagents are covered by a water-insoluble medium, then the DNA to be amplified is supplied and the water-insoluble medium is eliminated, so that the water-soluble reagents are dissolved and PCR can start. In the corresponding arrangement, a test unit designed as a single-use produce (a so-called cartridge) has at least one micro-channel or micro-cavity for receiving a PCR reagent. The PCR reagents in the form of a mixture which can be dried at a negligible vapour pressure and forms a storage-stable substance substance at room temperature adhere to the walls of the micro-channel or micro-cavity and form a thin film covered by an insoluble medium.