Directed enrichment of genomic DNA for high-throughput sequencing
US7993842B2 · kind B2 · utility
20Cited by
19References
39Claims
0Family size
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Key dates
| Filing date | Jul 16, 2009 |
| Grant date | Aug 9, 2011 |
| Priority date | — |
| Expiry date | Jul 16, 2029 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6837
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides microarrays of oligonucleotide primer pairs, and in particular, microarrays of primers that comprise at least one cleavable linkage. Also provided are methods to capture oligonucleotide primer pairs from one or more microarrays, and methods to use the captured oligonucleotide primer pairs, such as for amplification of a target polynucleotide sequence. In addition, methods of using a microarray to isolate, purify and/or amplify a target polynucleotide are provided.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.