Staphylococcus protein A domain mutants that bind to TNF-α
US7993848B2 · kind B2 · utility
2Cited by
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42Claims
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Key dates
| Filing date | Mar 1, 2006 |
| Grant date | Aug 9, 2011 |
| Priority date | — |
| Expiry date | Nov 2, 2027 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K14/31
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A TNF-α binding polypeptide is provided, which is related to a domain of staphylococcal protein A (SPA) in that the sequence of the polypeptide corresponds to the sequence of the SPA domain having 1 to about 20 substitution mutations. Nucleic acid encoding the polypeptide, expression vector comprising the nucleic acid, and host cell comprising the expression vector are also provided. Also provided are methods comprising a step of affinity separation or detection, in which step a polypeptide according to the invention is used. Such methods may be used for reducing the content of TNF-α in a body fluid.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.