Methods and apparatuses for sorting objects in forensic DNA analysis and medical diagnostics
US7998676B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Sep 11, 2008 |
| Grant date | Aug 16, 2011 |
| Priority date | — |
| Expiry date | Oct 29, 2028 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2001/007
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention relates to an apparatus and method of sorting objects and identifying the objects in a forensics sample, including using holographic optical trapping to sort objects from contaminants, and performing (single cell) PCR-based STR analysis on the objects to determine their identification. In addition, the chip used as a support for sorting the objects can also be used for performing single cell PCR-based STR analysis. In another embodiment, a microfluidics chip is used to stream the sample and sort the objects, before single cell PCR-based STR analysis is performed. The chip used for sorting utilizing HOT in the absence or presence of microfluidic streaming and sorting can also be the same as that used for the single cell PCR-based STR analysis.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.