Methods for conducting metabolic analyses
US8003076B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Jun 25, 2008 |
| Grant date | Aug 23, 2011 |
| Priority date | — |
| Expiry date | Oct 25, 2029 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/973
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention provides methods and apparatus for purifying metabolites of interest and conducting metabolic analyses. The methods generally involve determining metabolic flux values for a plurality of target analytes by monitoring the relative isotope abundance of a stable isotope in a substrate labeled with the stable isotope and/or one or more target metabolites formed through metabolism of the labeled substrate. Certain methods utilize multiple electrophoretic methods to separate the target analytes from other components within the sample being analyzed. The methods can be used in a variety of applications including screens to identify metabolites that are correlated with certain diseases and diagnostic screens for identifying individuals having, or susceptible to, a disease.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.