Multiplex cellular assays using detectable cell barcodes

US8003312B2 · kind B2 · utility

Assignee

• The Board of Trustees of the Leland Stanford Junior University · US

Inventors

• Peter O. Krutzik · Los Altos, US
• Garry P. Nolan · San Francisco, US

Key dates

• Filing date: Feb 14, 2008
• Grant date: Aug 23, 2011
• Expiry date: Feb 14, 2028

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2458/15
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

We describe herein a cell-based multiplexing technique called detectable cell barcoding (DCB). In DCB, each individual sample is labeled with a different DCB signature that distinguishes each sample by one or both of detected intensity or type of detection characteristic. The samples are then combined and analyzed for a detectable characteristic of interest (e.g., presence of an analyte). By employing multiple distinct DCB labels at varying concentrations, one can perform multiplex analyses on up to hundreds or thousands (or more) of cell samples in a single reaction tube. DCB reduces reagent consumption by factors of 100-fold or more, significantly reduces data acquisition times and allows for stringent control sample analysis.