Method of amplifying a target nucleic acid by rolling circle amplification
US8003322B2 · kind B2 · utility
1Cited by
1References
17Claims
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Key dates
| Filing date | Oct 8, 2008 |
| Grant date | Aug 23, 2011 |
| Priority date | — |
| Expiry date | Oct 8, 2028 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6855
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Provided is kit for and a method of amplifying a nucleic acid using rolling cyclic amplification (RCA), including amplifying a nucleic acid together with formation of a single-strand circular DNA template using RCA by reacting a reaction solution including: (a) two hairpin oligos, (b) a target nucleic acid, (c) a DNA ligase,(d) an endonuclease, (e) a DNA polymerase, and (f) a primer.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.