Purification of vaccinia viruses using hydrophobic interaction chromatography

US8003364B2 · kind B2 · utility

Assignees

• Bavarian Nordic A/S · DK
• Otto-Von-Guericke-Universität · DE
• Sartorius Stedim Biotech GmbH · DE

Inventors

• Sara Post Hansen · Hørsholm, DK
• Rene Faber · Oettingen, DE
• Udo Reichl · Magdeburg, DE
• Michael Wolff · Monheim, DE
• Anders Peter Gram

Key dates

• Filing date: Nov 20, 2009
• Grant date: Aug 23, 2011
• Expiry date: Nov 20, 2029

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2710/24151
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

The present invention relates to methods for purification of Vaccinia viruses (VV) and/or Vaccinia virus (VV) particles, which can lead to highly pure and stable virus preparations of predominantly biologically active viruses. The invention encompasses purifying a virus preparation in a sterilized way with high efficiency and desirable yield in terms of purity, biological activity and stability, aspects advantageous for industrial production.