Amplification vectors based on trans-splicing

US8003381B2 · kind B2 · utility

Assignee

• Icon Genetics GmbH · DE

Inventors

• Victor Klimyuk · Leipzig, DE
• Peter Ivanov · Moscow, RU
• Sylvestre Marillonnet · Bodenwerder, DE
• Meinhart Zenk · Halle (Saale), DE
• Yuri Gleba · Berlin, DE

Key dates

• Filing date: Apr 30, 2002
• Grant date: Aug 23, 2011
• Expiry date: Apr 30, 2022

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N15/8216
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

This invention provides a process of amplification and/or expression of a sequence of interest in a cell by providing for generating within the cell at least one amplicon by trans-splicing between an RNA sequence designed for being capable of trans-splicing and a target RNA, whereby the amplicon is capable of amplifying in the cell and capable of expressing a sequence of interest.