Buffer solution for electroporation and a method comprising the use of the same

US8003389B2 · kind B2 · utility

Assignee

• Lonza Cologne GmbH · DE

Inventors

• Gudula Riemen · Langenfeld, DE
• Elke Lorbach · Cologne, DE
• Juliana Helfrich · Glauchau, DE
• Gregor Siebenkotten · Frechen, DE
• Herbert Mueller-Hartmann · Cologne, DE
• Kirsten Rothmann-Cosic · Berlin, DE
• Corinna Thiel · Cologne, DE
• Meike Weigel
• Heike Wessendorf
• Helmut Brosterhus · Witzenhausen, DE
• Michael Nix

Key dates

• Filing date: Nov 9, 2006
• Grant date: Aug 23, 2011
• Expiry date: Apr 28, 2029

Classification

• Technology area (CPC A): Human Necessities
• CPC primary: A61K48/00
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

A method for introducing biologically active molecules into animal or human cells using electric current includes suspending the cells and dissolving the biologically active molecules in a buffer solution which has a buffer capacity of at least 20 mmol×l−1×pH−1 and an ionic strength of at least 200 mmol×l−1 at a change in the pH from pH 7 to pH 8 and at a temperature of 25° C. to form a suspension. The method further includes applying an electric voltage to the suspension so as to introduce the biologically active molecules into animal or human cells.