Patent · US Active

Measurement of vitamin D

US8003400B2 · kind B2 · utility

6Cited by

1References

9Claims

0Family size

Assignee

Roche Diagnostics Operations, Inc. · US

Inventors

Uwe Kobold · Wielenbach, DE
Thomas Duelffer · Weilheim, DE
Michael Grol · Feldafing, DE
Rupert Herrmann · Weilheim, DE
Herbert von der Eltz · Spaichingen, DE
Leopold Von Proff · Hohenpeißenberg, DE

Key dates

Filing date	Dec 3, 2008
Grant date	Aug 23, 2011
Priority date	—
Expiry date	Dec 3, 2028

Classification

Technology area (CPC Y)Emerging Cross-Sectional Technologies
CPC primaryY10T436/203332
WIPO fieldMeasurement
WIPO sectorInstruments

Abstract

The present invention relates to a method of measuring a vitamin D metabolite in a sample, the method comprising the steps of (a) treating said sample with a vitamin D metabolite releasing reagent under conditions appropriate to release a vitamin D metabolite from vitamin D-binding protein and not to cause protein precipitation, (b) subjecting the treated sample obtained in step (a) to a chromatographic separation, and (c) measuring a vitamin D metabolite during or after said chromatographic separation. The present invention also relates to methods for determining the vitamin D status of a subject, for use in the diagnosis of disease, and to agents and kits for use in performing the methods of the invention.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.