Methods for analyzing interactions between proteins and sugar chains
US8008094B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Dec 24, 2004 |
| Grant date | Aug 30, 2011 |
| Priority date | — |
| Expiry date | Jan 23, 2027 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/97
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
As a result of investigating the optimum conditions of methods for immobilizing proteins that interact with sugar chains onto a substrate, it was revealed that coating the surface of a slide glass with GTMS enables immobilization at a higher S/N ratio than conventionally possible. Moreover, by using a substrate to which a rubber with a number of holes was affixed to form a number of reaction vessels, and further by spotting lectins onto the substrate and washing with PBST, the weak interactions between sugar chains and lectins were successfully detected with improved sensitivity. In addition, by introducing an evanescent excitation-type scanner, it became possible to detect the interactions between lectins and sugar chains without washing away the probe solution.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.