Sequence diversity generation in immunoglobulins
US8012714B2 · kind B2 · utility
4Cited by
3References
44Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Apr 14, 2009 |
| Grant date | Sep 6, 2011 |
| Priority date | — |
| Expiry date | Apr 14, 2029 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2310/14
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Compositions and methods are disclosed for generating immunoglobulin structural diversity in vitro, and in particular, for reducing biases in V region and J segment gene utilization, and for generating immunoglobulin V-D-J recombination events in a manner that does not require D-J recombination to precede V-DJ recombination. Selection of advantageous combinations of immunoglobulin gene elements, including introduction of artificial diversity (D) segment genes and optimization of recombination signal sequence (RSS) efficiency, are disclosed.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.