Method for detecting and/or quantifying a known function from a nucleic acid sample
US8017318B1 · kind B1 · utility
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19Claims
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Key dates
| Filing date | Nov 28, 2000 |
| Grant date | Sep 13, 2011 |
| Priority date | — |
| Expiry date | Mar 3, 2025 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6897
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention concerns a method for detecting a known function, from nucleic acids present in a sample, characterized in that it comprises the following steps: (a) preparing, from the nucleic acids of the sample, nucleic acid molecules comprising the gene(s) coding for the protein(s) corresponding to said function, and the control elements required for the transcription and the translation of said gene(s); (b) in vitro transcription and translation of the nucleic acid molecule prepared in step (a); (c) detecting and/or measuring the function corresponding to the protein(s) produced in step (b).
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.