Patent · US Active

Reversible di-nucleotide terminator sequencing

US8017338B2 · kind B2 · utility

10Cited by
45References
11Claims
0Family size

Assignee

Inventor

Key dates

Filing dateNov 20, 2008
Grant dateSep 13, 2011
Priority date—
Expiry dateNov 20, 2028

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC12Q1/6869
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

The present teachings provide methods, compositions, and kits for synthesizing and sequencing nucleic acids. In some embodiments, reversible di-nucleotide compounds are employed along with cleaving reactions that remove a label and a blocking moiety. Improved sequencing efficiency is achieved by the rapid polymerase-mediated incorporation of reversible di-nucleotide compounds. In some embodiments, the di-nucleotides do not contain conventional nucleotide triphosphates, but rather employ amino acid phosphoramidate nucleotides (AAPNs).

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.