Compositions and kits for recombinase polymerase amplification
US8017339B2 · kind B2 · utility
66Cited by
17References
19Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jun 14, 2010 |
| Grant date | Sep 13, 2011 |
| Priority date | — |
| Expiry date | Jun 14, 2030 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/70
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
This disclosure describes three related novel methods for Recombinase-Polymerase Amplification (RPA) of a target DNA that exploit the properties of recombinase and related proteins, to invade double-stranded DNA with single stranded homologous DNA permitting sequence specific priming of DNA polymerase reactions. The disclosed methods have the advantage of not requiring thermocycling or thermophilic enzymes. Further, the improved processivity of the disclosed methods may allow amplification of DNA up to hundreds of megabases in length.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.