Enzymatic hydrolysis of lignocellulosic feedstocks using accessory enzymes

US8017361B2 · kind B2 · utility

Assignee

• Iogen Energy Corporation · CA

Inventors

• Brian R. Scott · Richmond, CA
• Christopher Hill · Ottawa, CA
• John J. Tomashek · Schreiber, CA
• Chengsong Liu · Schreiber, CA

Key dates

• Filing date: Aug 29, 2008
• Grant date: Sep 13, 2011
• Expiry date: Aug 27, 2029

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y02E50/10
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Provided is an enzyme mixture for hydrolyzing a pretreated lignocellulosic feedstock to soluble sugars. The enzyme mixture comprises EG4 at a fractional concentration (fEG4) of about 0.25 to about 0.83 (w/w), Swollenin at a fractional concentration (fSwo1) of about 0 to about 0.66 (w/w), and Cip1 at a fractional concentration (fCip1) of 0 to about 0.33 measured relative to all accessory enzymes present in the enzyme mixture. Also provided are processes for converting a pretreated lignocellulosic feedstock to soluble sugars using the enzyme mixtures, and methods of using and producing such enzyme mixtures.