Method for chondrocyte expansion with phenotype retention
US8017394B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Sep 21, 2007 |
| Grant date | Sep 13, 2011 |
| Priority date | — |
| Expiry date | Feb 3, 2030 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2533/80
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides a method for expanding a population of chondrocytes that maintains chondrocyte phenotype during the expansion by culturing the population in a defined serum-free expansion medium containing one or more cytokines and under low attachment conditions. The method further solves diffusion problems during the subsequent stage of extracellular matrix production by use of a perforated polycarbonate substrate that results in a randomly organized cultured neocartilage tissue. Chondrocytes expanded and cultured in this manner can be used in various medical applications to repair cartilaginous tissues that have been injured by trauma or disease.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.