Method for transformation using mutant acetolactate synthase gene
US8017400B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | May 9, 2006 |
| Grant date | Sep 13, 2011 |
| Priority date | — |
| Expiry date | Mar 23, 2028 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/8274
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Transformed cells are efficiently selected using a mutant ALS gene having high specificity to PC herbicides. The transformation method comprises the steps of: transforming a host cell with a recombination vector containing a gene of interest and a gene coding for a mutant acetolactate synthase having mutation of glycine corresponds to position 95 of the amino acid sequence of a wild-type acetolactate synthase derived from rice to alanine; culturing the transformed cell obtained in the former step in the presence of a pyrimidinyl carboxy herbicide; and wherein the gene coding for the mutant acetolactate synthase is used as a selection marker.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.