Apparatus and method for identifying peaks in liquid chromatography/mass spectrometry data and for forming spectra and chromatograms
US8017908B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Dec 18, 2009 |
| Grant date | Sep 13, 2011 |
| Priority date | — |
| Expiry date | Mar 17, 2030 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG06F2218/08
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Chromatograms and mass spectra produced by an LC/MS system are analyzed by creating a two-dimensional data matrix of the spectral and chromatographic data. The two-dimensional matrix can be created by placing the spectra generated by the mass spectrometer portion of the LC/MS system in successive columns of the data matrix. In this way, the rows of the data matrix correspond to chromatographic data and the columns of the data matrix correspond to the spectra. A two-dimensional filter is specified and applied to the data matrix to enhance the ability of the system to detect peaks associated with ions. The two-dimensional filter is specified according to desired criteria. Rank-1 and rank-2 filters can be specified to improve computational efficiency. One method of applying the two-dimensional filter is through convolution of the data matrix with the two-dimensional filter to produce an output data matrix. Peaks corresponding to detected ions are identified in the output data matrix. Parameters of the peaks are determined and stored for later processing including quantitation, or simplification of chromatograms or spectra by, for example, identifying peaks associating with ions having…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.