Hydroxyapatite-binding peptides for bone growth and inhibition

US8022040B2 · kind B2 · utility

Assignee

• The Regents of the University of California · US

Inventors

• Carolyn R. Bertozzi · Berkeley, US
• Jie Song · Beijing, CN
• Seung-Wuk Lee · Walnut Creek, US

Key dates

• Filing date: Nov 29, 2005
• Grant date: Sep 20, 2011
• Expiry date: Jan 22, 2026

Classification

• Technology area (CPC A): Human Necessities
• CPC primary: A61K38/00
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Hydroxyapatite (HA)-binding peptides are selected using combinatorial phage library display. Pseudo-repetitive consensus amino acid sequences possessing periodic hydroxyl side chains in every two or three amino acid sequences are obtained. These sequences resemble the (Gly-Pro-Hyp)x repeat of human type I collagen, a major component of extracellular matrices of natural bone. A consistent presence of basic amino acid residues is also observed. The peptides are synthesized by the solid-phase synthetic method and then used for template-driven HA-mineralization. Microscopy reveal that the peptides template the growth of polycrystalline HA crystals ˜40 nm in size.