Expression of biologically active polypeptides in duckweed
US8022270B2 · kind B2 · utility
11Cited by
17References
11Claims
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Key dates
| Filing date | Sep 30, 2003 |
| Grant date | Sep 20, 2011 |
| Priority date | — |
| Expiry date | Aug 18, 2026 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2317/55
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods, nucleic acid sequences, and transformed duckweed plant or duckweed nodule cultures for the expression and the secretion of biologically active polypeptides from genetically engineered duckweed are provided. Expression of recombinant polypeptides in duckweed is improved by modifying the nucleotide sequence of the expression cassette encoding the polypeptide for improved expression in duckweed. Recovery of biologically active polypeptides from duckweed is improved by linking the biologically active polypeptide to a signal peptide that directs the secretion of the polypeptide into the culture medium.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.