Detection of biotargets using bioreceptor functionalized nanoparticles
US8026108B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Oct 19, 2007 |
| Grant date | Sep 27, 2011 |
| Priority date | — |
| Expiry date | Dec 17, 2028 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/54346
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
An ultra sensitive method for detection of biomolecules includes the step of providing a plurality of bioreceptor functionalized nanoparticle probes. The nanoparticles can include metal, semiconductor, radioactive isotope or fluorescent dye molecules. A sample solution suspected of including the target is contacted with the probes, wherein if present, the target binds to the bioreceptor. After such binding a separating step follows. In the separating step, probes having the target bound thereto are separated from probes not having the target bound thereto. In one embodiment probes having the target bound thereto are then decomposed to generate ions, or broken into discrete radioactive isotopes or fluorescent dye molecules to form a solution including a large plurality of metal ions, radioactive isotopes or dye molecules. A concentration of ions, radioactive isotopes, or dye molecules in the solution is then determined and using this information the concentration of the target in the original sample solution is determined.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.