Method for determining the substrate specificity of an enzyme

US8029979B2 · kind B2 · utility

Assignee

• JPT Peptide Technologies GmbH · DE

Inventors

• Jens Schneider-Mergener · Berlin, DE
• Mike Schutkowski · Nebra (Unstrut), DE
• Ulf Reimer · Berlin, DE
• Liying Dong · Berlin, DE
• Soren Panse · Berlin, DE
• Dirk Scharn · Wien, AT
• Frank Osterkamp · Berlin, DE
• Gerd Hummel · Berlin, DE
• Laurence Jobron · Berlin, DE

Key dates

• Filing date: Jun 26, 2006
• Grant date: Oct 4, 2011
• Expiry date: Jun 26, 2026

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/485
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The invention relates to a method for determining the substrate specificity of an enzymatic activity comprising the following steps: providing an assembly comprising a plurality of amino acid sequences on a planar surface of a support material, whereby the amino acids are directionally immobilized; contacting and/or incubating of an enzymatic activity with the assembly; and detection of a reaction between one of the amino acid sequences that are immobilized on the assembly and the enzymatic activity. According to the invention, during the reaction of the enzymatic activity with the assembly, a change in the molecular weight of at least one of the amino acid sequences takes place.