Oligonucleotide mediated nucleic acid recombination

US8029988B2 · kind B2 · utility

Assignee

• Codexis Mayflower Holdings, Inc. · US

Inventors

• Andreas Crameri · Los Altos Hills, US
• Willem P. C. Stemmer · Los Gatos, US
• Jeremy Minshull · Los Altos, US
• Steven H. Bass · Hillsborough, US
• Mark Welch · Fremont, US
• Jon E. Ness · Sunnyvale, US
• Claes Gustafsson · Belmont, US
• Phillip A. Patten · Portola Valley, US

Key dates

• Filing date: Nov 30, 2007
• Grant date: Oct 4, 2011
• Expiry date: Feb 4, 2029

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12P21/06
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Methods of recombining nucleic acids, including homologous nucleic acids, are provided. Families of gene shuffling oligonucleotides and their use in recombination procedures, as well as polymerase and ligase mediated recombination methods are also provided.