Patent · US Active

Thermostable luciferases and methods of production

US8030017B2 · kind B2 · utility

29Cited by

55References

11Claims

0Family size

Assignee

Promega Corporation · US

Inventors

Keith V. Wood · Verona, US
Monika G. Wood · Verona, US
Mary Hall · Waunakee, US

Key dates

Filing date	Jul 31, 2009
Grant date	Oct 4, 2011
Priority date	—
Expiry date	Jul 31, 2029

Classification

Technology area (CPC Y)Emerging Cross-Sectional Technologies
CPC primaryY10S977/898
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

Luciferase enzymes with greatly increased thermostability, e.g., at least half lives of 2 hours at 50° C., cDNAs encoding the novel luciferases, and hosts transformed to express the luciferases, are disclosed. Methods of producing the luciferases include recursive mutagenesis. The luciferases are used in conventional methods, some employing kits.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.