Substitutional variants of hydroxynitrile lyase with high specific activity and methods of use
US8030053B2 · kind B2 · utility
3Cited by
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30Claims
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Key dates
| Filing date | Oct 14, 2005 |
| Grant date | Oct 4, 2011 |
| Priority date | — |
| Expiry date | Sep 16, 2028 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12P13/004
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
An improved hydroxynitrile lyase characterized by having a mutation of substitution of at least one amino acid residue in the amino acid sequence of a wild-type hydroxynitrile lyase with another amino acid and by its hydroxynitrile lyase activity per transformant being higher than the hydroxynitrile lyase activity per transformant into which the wild-type hydroxynitrile lyase gene is introduced; and a method for producing a hydroxynitrile lyase, comprising expressing the improved hydroxynitrile lyase in a host and recovering the improved hydroxynitrile lyase from the resultant culture.
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