Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites

US8030066B2 · kind B2 · utility

Assignee

• Life Technologies Corporation · US

Inventors

• Jonathan Chesnut · Carlsbad, US
• John Carrino · San Diego, US
• Louis Leong · Ocean City, US
• Knut Madden · Carlsbad, US
• Martin Gleeson · San Diego, US
• James Fan · Carlsbad, US
• Michael A. Brasch · Gaithersburg, US
• David Cheo · Kensington, US
• James L. Hartley · Frederick, US
• Devon Byrd · Germantown, US
• Gray F. Temple · Grass Valley, US

Key dates

• Filing date: Dec 18, 2006
• Grant date: Oct 4, 2011
• Expiry date: Oct 4, 2028

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Y599/01
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention provides compositions and methods for recombinational cloning. The compositions include vectors having multiple recombination sites and/or multiple topoisomerase recognition sites. The methods permit the simultaneous cloning of two or more different nucleic acid molecules. In some embodiments the molecules are fused together while in other embodiments the molecules are inserted into distinct sites in a vector. The invention also generally provides for linking or joining through recombination a number of molecules and/or compounds (e.g., chemical compounds, drugs, proteins or peptides, lipids, nucleic acids, carbohydrates, etc.) which may be the same or different. The invention also provides host cells comprising nucleic acid molecules of the invention or prepared according to the methods of the invention, and also provides kits comprising the compositions, host cells and nucleic acid molecules of the invention, which may be used to synthesize nucleic acid molecules according to the methods of the invention.