Monoclonal antibodies to tacrolimus and immunoassays methods for tacrolimus

US8030458B2 · kind B2 · utility

Assignee

• Siemens Healthcare Diagnostics Inc. · US

Inventors

• Kenneth Cornell Kasper · Monte Sereno, US
• Henry J. Jeong · Palo Alto, US
• Dariush Davalian · San Jose, US
• Hshiou-ting Liu · Milpitas, US
• Paul Levi Miller · South San Francisco, US
• Denise Williams · San Jose, US

Key dates

• Filing date: Sep 26, 2008
• Grant date: Oct 4, 2011
• Expiry date: Aug 6, 2029

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S436/815
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

An IgG1λ monoclonal antibody to the immunosuppressive drug tacrolimus has improved properties. In particular, this monoclonal antibody, designated 1H6, has reduced cross-reactivity to several tacrolimus metabolites. This antibody is suitable for performance of immunoassays such as homogeneous immunoassays to detect or determine the presence or concentration of tacrolimus in samples such as blood samples. The invention further includes derivatives of tacrolimus derivatized at a non-binding portion of the molecule useful in immunizing antibody-producing animals and in producing such monoclonal antibodies, as well as labeled derivatives of tacrolimus useful as tacrolimus analogues in such assays. The invention further includes immunoassay methods for the detection of tacrolimus and test kits useful in performing such immunoassays.