Enhanced sequencing by hybridization using pools of probes

US8034566B2 · kind B2 · utility

Assignee

• Callida Genomics, Inc. · US

Inventors

• Radoje Drmanac · Los Altos Hills, US
• Snezana Drmanac · Nanhu, CN
• David Kita · Milpitas, US
• Cory Cooke · San Mateo, US
• Chongjun Xu · San Jose, US

Key dates

• Filing date: Oct 30, 2007
• Grant date: Oct 11, 2011
• Expiry date: Jun 3, 2029

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6874
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The invention provides methods for sequencing by hybridization (SBH) using pools of probes that allow greater efficiency in conducting SBH by reducing the number of separate measurements of hybridization signals required to identify each particular nucleotide in a target nucleic acid sequence. The invention also provides pools and sets of pools of probes, as well as methods of generating pools of probes.