Isothermal nucleic acid amplification methods and compositions
US8034568B2 · kind B2 · utility
24Cited by
173References
40Claims
0Family size
Assignee
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Key dates
| Filing date | Feb 12, 2009 |
| Grant date | Oct 11, 2011 |
| Priority date | — |
| Expiry date | Feb 12, 2029 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/686
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods and compositions are provided related to the amplification of target polynucleotide sequences as well as total RNA and total DNA amplification. In some embodiments, the methods and compositions also allow for the immobilization and capture of target polynucleotides with defined 3′ and or 5′ sequences to solid surfaces. The polynucleotides attached to the solid surfaces can be amplified or eluted for downstream processing. In some cases, nucleotides attached to solid surfaces can be used for high throughput sequencing of nucleotide sequences related to target DNA or target RNA.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.