Tagged oligonucleotides and their use in nucleic acid amplification methods
US8034570B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Sep 28, 2010 |
| Grant date | Oct 11, 2011 |
| Priority date | — |
| Expiry date | Sep 28, 2030 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6895
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Disclosed are methods for selective amplification of target nucleic acid sequence using a tagged oligonucleotide comprising a target hybridizing sequence that hybridizes to a 3′-end of the target nucleic acid and a tag sequence situated 5′ to the target hybridizing sequence. The tagged oligonucleotide is hybridized to the target nucleic acid and, after reducing the effective concentration of unhybridized tagged oligonucleotide having an active form, a primer extension reaction is initiated to produce a primer extension product. Further amplification also utilizes a first oligonucleotide that hybridizes to the 3′ end of the complement of the target nucleic acid and a second oligonucleotide that hybridizes to the complement of the tag sequence. Also disclosed are reaction mixtures for use in the disclosed methods comprising the tagged oligonucleotide hybridized to target nucleic and substantially free of an active form of unhybridized tagged oligonucleotide.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.