Methods of enhanced heterologous protein secretion
US8034607B2 · kind B2 · utility
5Cited by
8References
17Claims
0Family size
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Key dates
| Filing date | Sep 12, 2008 |
| Grant date | Oct 11, 2011 |
| Priority date | — |
| Expiry date | Apr 21, 2029 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/81
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method of enhancing heterologous protein secretion in a yeast cell is disclosed. In one embodiment, the method comprising the steps of engineering a yeast cell to overexpress at last one gene selected from the group consisting of CCW12, CWP2, SED1, RPP0, ERO1 and their homologs, supplying the yeast cell with a nucleic acid encoding a heterologous protein and obtaining increased expression of the heterologous protein, wherein the expression is increased relative to the protein expression in a yeast cell that does not overexpress a gene selected from the group consisting of CCW12, CWP2, SED1, RPP0, ERO1 and their homologs.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.