Ultra-sensitive magnetoreduction measurement system and ultra-sensitive, wash-free assay using the same
US8034634B2 · kind B2 · utility
Inventors
Key dates
| Filing date | Oct 4, 2007 |
| Grant date | Oct 11, 2011 |
| Priority date | — |
| Expiry date | Oct 7, 2029 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/54326
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
An ultra-sensitive, wash-free method for quantitatively determining the concentration of biomolecules in a sample solution is provided. The sensitivity of the method is 1 ppt or below. The method includes providing a reagent containing magnetic nanoparticles and measuring the ac magnetic susceptibility of the reagent. Then, a sample solution containing either multiple-active epitope biomolecules or single-active-epitope biomolecules is mixed with the reagent. The ac magnetic susceptibility of the reagent after the association with the biomolecules is measured. Thereafter, the difference in the ac magnetic susceptibility of the reagent before and after the association with the biomolecules is measured and the concentration of biomolecules in the sample solution is determined.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.