Buffer solution for electroporation and a method comprising the use of the same

US8039259B2 · kind B2 · utility

Assignee

• Lonza Cologne GmbH · DE

Inventors

• Gudula Riemen · Langenfeld, DE
• Elke Lorbach · Cologne, DE
• Juliana Helfrich · Glauchau, DE
• Gregor Siebenkotten · Frechen, DE
• Herbert Mueller-Hartmann · Cologne, DE
• Kirsten Rothmann-Cosic · Berlin, DE
• Corinna Thiel · Cologne, DE
• Meike Weigel
• Heike Wessendorf
• Helmut Brosterhus · Witzenhausen, DE
• Michael Nix

Key dates

• Filing date: Oct 31, 2007
• Grant date: Oct 18, 2011
• Expiry date: Oct 12, 2029

Classification

• Technology area (CPC A): Human Necessities
• CPC primary: A61K48/00
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

A buffer solution for suspending animal or human cells and for dissolving biologically active molecules in order to introduce the biologically active molecules into the cells using electric current. The buffer solution includes at least one of sodium succinate, mannitol and sodium lactobionate. The buffer solution has a buffer capacity of at least 20 mmol*l−1*pH−1 at a change in the from pH 7 to pH 8 and at a temperature of 25° C., and an ionic strength of at least 200 mmol * l−1.