Hyaluronic acid binding peptides enhance host defense against pathogenic bacteria

US8044022B2 · kind B2 · utility

Inventors

• Tadeusz Kolodka · Cross Lake, CA
• Bernard T. Charlton · Cross Lake, CA
• Wendy Johnson · Ealing, GB

Key dates

• Filing date: Jun 8, 2006
• Grant date: Oct 25, 2011
• Expiry date: Mar 11, 2028

Classification

• Technology area (CPC A): Human Necessities
• CPC primary: A61K38/00
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Several species of bacteria capable of invasive infections, such as S. pyogenes, S. equi and P. multocida, contain hyaluronic acid (HA) in their capsules. Bacterial species such as Staphylococcus aureus and related Staphylococci have capsules that contain acidic polysaccharides. Bacterial capsule or bacterial surface binding peptides were synthesized and tested in a culture model of invasive bacterial infections, specifically translocation through polarized keratinocyte cultures. The peptides reduced the translocation of a variety of bacterial species, with a concomitant increase in bacterial internalization by the keratinocytes. In vivo, subcutaneous inoculation of encapsulated GAS treated with peptides delayed bacterial dissemination. In a mouse surgical wound model infected with S. aureus, treatment with peptides reduced the numbers of bacteria and inflammation at the wound site.