Methods for the preparation of lysine by fermentation of Corynebacterium glutamicum

US8048651B2 · kind B2 · utility

Assignee

• BASF SE

Inventors

• Oskar Zelder · Speyer, DE
• Corinna Klopprogge · Mannheim, DE
• Hartwig Schröder · Nümbrecht, DE
• Stefan Haefner · Speyer, DE
• Burkhard Kroger · Schifferstadt, DE
• Patrick Kiefer · Bellinzona, CH
• Elmar Heinzle · Riegelsberg, DE
• Christoph Wittmann · Saarlouis, DE

Key dates

• Filing date: Jul 31, 2009
• Grant date: Nov 1, 2011
• Expiry date: Jul 31, 2029

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N9/16
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention features methods of increasing the production of a fine chemical, e.g., lysine from a microorganism, e.g., Corynebacterium by way of deregulating an enzyme encoding gene, i.e., fructose-1,6-bisphosphatase. In a preferred embodiment, the invention provides methods of increasing the production of lysine in Corynebacterium glutamicum by way of increasing the expression of fructose-1,6-bisphosphatase activity. The invention also provides a novel process for the production of lysine by way of regulating carbon flux towards oxaloacetate (OAA). In a preferred embodiment, the invention provides methods for the production of lysine by way of utilizing fructose or sucrose as a carbon source.