Taxus transformation transformed cells, and related compositions and methods
US8053637B2 · kind B2 · utility
0Cited by
7References
19Claims
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Key dates
| Filing date | Jan 3, 2007 |
| Grant date | Nov 8, 2011 |
| Priority date | — |
| Expiry date | Apr 3, 2028 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/8205
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Reported herein are methods for obtaining stably transformed callus in Taxus media ‘Hicksii’, including particularly methods that involve using needles, stem, or bark peel as explant material for transformation. Also provided are descriptions of several promoter activities in directing reporter gene expression in Taxus media cells, in particular cells in suspension cultures, callus and needles. Transgenic plants (e.g., Taxus plants), plant cells, cell lines, and tissues (including seeds) are also provided, in particular those that express one or more enzymes in a paclitaxel biosynthesis pathway.
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