Method for estimating a melting temperature of a nucleic acid in buffers containing magnesium ions
US8055451B2 · kind B2 · utility
2Cited by
2References
27Claims
0Family size
Assignee
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Key dates
| Filing date | Jan 7, 2008 |
| Grant date | Nov 8, 2011 |
| Priority date | — |
| Expiry date | Aug 4, 2029 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG16B20/00
- WIPO fieldComputer technology
- WIPO sectorElectrical engineering
Abstract
The invention relates to methods and systems for predicting or estimating the melting temperature of duplex nucleic acids, in the presence of divalent cations, particularly duplexes of oligonucleotides which may be used as, for example, but not limited to primers or probes in PCR and/or hybridization assays. The methods and algorithms use novel formulas, having terms and coefficients that are functions of the particular nucleotide sequence, to estimate the effect of divalent cation salt conditions on the melting temperature.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.