Label-free optical sensing and characterization of biomolecules by d8 or d10 metal complexes
US8057989B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jan 19, 2007 |
| Grant date | Nov 15, 2011 |
| Priority date | — |
| Expiry date | Sep 16, 2030 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/52
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention provides a composition for detecting and/or characterizing a multiple-charged biomolecule comprising a charged d8 or d10 metal complex, wherein the metal complex electrostatically binds to the multiple-charged biomolecule to induce aggregation and self-assembly of the metal complex through metal . . . metal interactions, π . . . π interactions, or a combination of both interactions. The present invention further provides assay methods and kits for label-free optical detection and/or characterization of biomolecules carrying multiple charges, e.g., single-stranded nucleic acids, polyaspartate, polyglutamate, using a composition comprising a charged d8 or d10 metal complex.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.